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Thursday, July 28, 2011

Analysis of Bronchoaveolar Lavage Fluid in Cats


Bronchoalveolar lavage fluid (BALF) collection is an important diagnostic tool for cats with respiratory disease, especially lower respiratory tract disease. The accurate cytological evaluation of BALF is a useful means of differentiating disease processes. The procedure can also be used as a means to monitor the progression of disease or the response to therapy. The effects of storage on BALF values of total nucleated cell counts (TNCC) and differential cell counts (DCC), cell morphology and cytological diagnosis were evaluated. Samples from 45 research cats with neutrophilic, eosinophilic, and mixed inflammation and healthy controls were utilized for the study. The BALF samples were evaluated at 1 hour, or stored at 4 C° or room temperature for 24 hours, or 4 C° or room temperature for 48 hours. Storage of feline BALF at various time and temperature conditions alters TNCC and DCC, cellular morphology, and cytological diagnosis. There was a significant decrease in BALF TNCC in samples stored at room temperature for 48 hours. There was also an increase in the percentage of BALF eosinophils following storage at room temperature for 24 and 48 hours. The authors noted that it is important to remember that the relative increase in percentage of eosinophils is the result in a decrease in other cell lines due to cellular apoptosis accelerated with the storage at room temperature. Following storage, there was a misclassification of the cytological analysis of BALF from inflammatory airway disease to non-inflammatory in 9-18% of the samples. Many BALF samples (31-57%) had a change in cytological diagnosis following storage, similarly rising along with the increase in storage time and temperature. Ideally, analysis of feline BALF should be performed promptly after collection.  [VT]

Related articles:
Nafe L, Declue AE, Lee-Fowler TM, et al. Evaluation of biomarkers in bronchoalveolar lavage fluid for discrimination between asthma and chronic bronchitis in cats. Am J Vet Res 2010;71:583-591.


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Bronchoalveolar lavage fluid (BALF) collection is an important diagnostic tool for cats with respiratory disease, especially lower respiratory tract disease. The accurate cytological evaluation of BALF is a useful means of differentiating disease processes. The procedure can also be used as a means to monitor the progression of disease or the response to therapy. The effects of storage on BALF values of total nucleated cell counts (TNCC) and differential cell counts (DCC), cell morphology and cytological diagnosis were evaluated. Samples from 45 research cats with neutrophilic, eosinophilic, and mixed inflammation and healthy controls were utilized for the study. The BALF samples were evaluated at 1 hour, or stored at 4 C° or room temperature for 24 hours, or 4 C° or room temperature for 48 hours. Storage of feline BALF at various time and temperature conditions alters TNCC and DCC, cellular morphology, and cytological diagnosis. There was a significant decrease in BALF TNCC in samples stored at room temperature for 48 hours. There was also an increase in the percentage of BALF eosinophils following storage at room temperature for 24 and 48 hours. The authors noted that it is important to remember that the relative increase in percentage of eosinophils is the result in a decrease in other cell lines due to cellular apoptosis accelerated with the storage at room temperature. Following storage, there was a misclassification of the cytological analysis of BALF from inflammatory airway disease to non-inflammatory in 9-18% of the samples. Many BALF samples (31-57%) had a change in cytological diagnosis following storage, similarly rising along with the increase in storage time and temperature. Ideally, analysis of feline BALF should be performed promptly after collection.  [VT]

Related articles:
Nafe L, Declue AE, Lee-Fowler TM, et al. Evaluation of biomarkers in bronchoalveolar lavage fluid for discrimination between asthma and chronic bronchitis in cats. Am J Vet Res 2010;71:583-591.


More on cat health: Winn Feline Foundation Library
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Follow us on Twitter
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Monday, July 25, 2011

Feline Cytauxzoonosis


Cytauxzoonosis is an acute-onset disease of cats caused by the parasite Cytauxzoon felis, which is transmitted by the bite of a tick. The disease is historically considered a fatal infection in domestic cats, though a number of cats have been known to have survived the disease. Most affected cats are young adults with exposure to the outdoors and vague clinical signs of lethargy and anorexia. The standard therapy for cytauxzoonosis has become imidocarb diproprionate. A combination of atovaquone and azithromycin (A&A) has also been suggested as a treatment.  Neither form of therapy has been prospectively evaluated for efficacy. Eighty acutely ill cats with Cytauxzoon felis infection were treated at one of 18 veterinary clinics.  Of 53 cats treated with A&A, 32 (60%) survived to discharge. Only 7 of 27 cats (26%) treated with imidocarb survived. Still, mortality remained high with approximately 40% of A&A treated cats dying in the hospital. The course of illness is swift and most cats that died did so shortly after presentation for care. The authors suggest that efforts must remain focused on prevention of disease, either through minimization of exposure to tick vectors or through development of chemoprophylaxis or vaccination. [VT]

Related articles:

Cytauxzoonosis is an acute-onset disease of cats caused by the parasite Cytauxzoon felis, which is transmitted by the bite of a tick. The disease is historically considered a fatal infection in domestic cats, though a number of cats have been known to have survived the disease. Most affected cats are young adults with exposure to the outdoors and vague clinical signs of lethargy and anorexia. The standard therapy for cytauxzoonosis has become imidocarb diproprionate. A combination of atovaquone and azithromycin (A&A) has also been suggested as a treatment.  Neither form of therapy has been prospectively evaluated for efficacy. Eighty acutely ill cats with Cytauxzoon felis infection were treated at one of 18 veterinary clinics.  Of 53 cats treated with A&A, 32 (60%) survived to discharge. Only 7 of 27 cats (26%) treated with imidocarb survived. Still, mortality remained high with approximately 40% of A&A treated cats dying in the hospital. The course of illness is swift and most cats that died did so shortly after presentation for care. The authors suggest that efforts must remain focused on prevention of disease, either through minimization of exposure to tick vectors or through development of chemoprophylaxis or vaccination. [VT]

Related articles:
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Sunday, July 24, 2011

The Onion, a Natural Alternative to Artificial Preservatives

Some components of the onion have antioxidant and antimicrobial properties, making it possible to use this bulb for food preservation. This is demonstrated by researchers from the Polytechnic University of Cataluña (UPC) and the University of Barcelona (UB) in a study that has just been published in the International Journal of Food Science and Technology.

"The antioxidant and antimicrobial properties of the flavonoids of the raw onion make it a good candidate for use in food preservation," says researcher Jonathan Santas from the Department of Nutrition and Bromatology at UB and co-author of a project carried out in the Department of Agrifood Engineering and Biotechnology at UPC.

The study, that has just been published by the International Journal of Food Science and Technology, shows that the flavonoids of onion, in addition to having beneficial properties for health, increase the life of foods, and so "they are a natural alternative to artificial additives used in the food industry." Flavonoids are phenolic compounds (with the phenol group) which are synthesized by plants.

The results confirm that, especially the yellow variety, is "a good source of these types of substances, and there is a positive correlation between the presence of flavonoids and their antioxidant capacity."

"The onion can be effective for delaying lipid oxidation in emulsions of oil and water -a model system of foods like margarines and mayonnaises-, and it also inhibits the growth of microorganisms that alter foods," Santas indicates.

The scientific team analysed onions of the White varieties "Fuentes de Ebro" and "Calçot de Valls" and the yellow variety "Grano de Oro." Using them the researchers demonstrated that phenolic compounds in the onion prevent the development of bacteria such as Bacillus cereus, Staphylococcus aureus, Micrococcus luteus and Listeria monocytogenes, microorganisms typically associated with the deterioration of foods.

Previous studies indicate that flavonoids have beneficial effects for health due to their antioxidant, anti-inflammatory, cardioprotective, vasodilatory and anti-carcinogenic properties, making it of special interest in the prevention of chronic illnesses, such as cardiovascular illnesses, and some types of cancer.


A more stable component


The flavonoids of the onion are more stable than some of its other components, such as sulphur compounds. Traditionally it was indicated that these sulphuric compounds are good for the health, as they are responsible for the characteristic taste, aroma and lacrimogenic effects of the plant. These substances, which are very volatile and unstable, are released when the onion is damaged or cut.

The onion (Allium cepa) is one of the most cultivated and consumed vegetables on the planet (around 66 million tonnes in 2008, of which 1.1 million were produced in Spain, especially in Castilla-La Mancha), and one of the main ingredients of the Mediterranean diet.
Some components of the onion have antioxidant and antimicrobial properties, making it possible to use this bulb for food preservation. This is demonstrated by researchers from the Polytechnic University of Cataluña (UPC) and the University of Barcelona (UB) in a study that has just been published in the International Journal of Food Science and Technology.

"The antioxidant and antimicrobial properties of the flavonoids of the raw onion make it a good candidate for use in food preservation," says researcher Jonathan Santas from the Department of Nutrition and Bromatology at UB and co-author of a project carried out in the Department of Agrifood Engineering and Biotechnology at UPC.

The study, that has just been published by the International Journal of Food Science and Technology, shows that the flavonoids of onion, in addition to having beneficial properties for health, increase the life of foods, and so "they are a natural alternative to artificial additives used in the food industry." Flavonoids are phenolic compounds (with the phenol group) which are synthesized by plants.

The results confirm that, especially the yellow variety, is "a good source of these types of substances, and there is a positive correlation between the presence of flavonoids and their antioxidant capacity."

"The onion can be effective for delaying lipid oxidation in emulsions of oil and water -a model system of foods like margarines and mayonnaises-, and it also inhibits the growth of microorganisms that alter foods," Santas indicates.

The scientific team analysed onions of the White varieties "Fuentes de Ebro" and "Calçot de Valls" and the yellow variety "Grano de Oro." Using them the researchers demonstrated that phenolic compounds in the onion prevent the development of bacteria such as Bacillus cereus, Staphylococcus aureus, Micrococcus luteus and Listeria monocytogenes, microorganisms typically associated with the deterioration of foods.

Previous studies indicate that flavonoids have beneficial effects for health due to their antioxidant, anti-inflammatory, cardioprotective, vasodilatory and anti-carcinogenic properties, making it of special interest in the prevention of chronic illnesses, such as cardiovascular illnesses, and some types of cancer.


A more stable component


The flavonoids of the onion are more stable than some of its other components, such as sulphur compounds. Traditionally it was indicated that these sulphuric compounds are good for the health, as they are responsible for the characteristic taste, aroma and lacrimogenic effects of the plant. These substances, which are very volatile and unstable, are released when the onion is damaged or cut.

The onion (Allium cepa) is one of the most cultivated and consumed vegetables on the planet (around 66 million tonnes in 2008, of which 1.1 million were produced in Spain, especially in Castilla-La Mancha), and one of the main ingredients of the Mediterranean diet.
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Effect of dietary krill oil supplementation on the endocannabinoidome of metabolically relevant tissues from high fat-fed mice.

ABSTRACT: BACKGROUND: Omega-3 polyunsaturated fatty acids (omega-3-PUFA) are known to ameliorate several metabolic risk factors for cardiovascular disease, and an association between elevated peripheral levels of endogenous ligands of cannabinoid receptors (endocannabinoids) and the metabolic syndrome has been reported. We investigated the dose-dependent effects of dietary omega-3-PUFA supplementation, given as krill oil (KO), on metabolic parameters in high fat diet (HFD)-fed mice and, in parallel, on the levels, in inguinal and epididymal adipose tissue (AT), liver, gastrocnemius muscle, kidneys and heart, of: 1) the endocannabinoids, anandamide and 2-arachidonoylglycerol (2-AG), 2) two anandamide congeners which activate PPARalpha but not cannabinoid receptors, N-oleoylethanolamine (OEA) and N-palmitoylethanolamine (PEA), and 3) the direct biosynthetic precursors of these compounds. Methods: Lipids were identified and quantified using liquid chromatography coupled to atmospheric pressure chemical ionization single quadrupole mass spectrometry (LC-APCI- MS) or high resolution ion trap-time of flight mass spectrometry (LC-IT-ToF-MS).

Eight-week HFD increased endocannabinoid levels in all tissues except the liver and epididymal AT, and KO reduced anandamide and/or 2-AG levels in all tissues but not in the liver, usually in a dose-dependent manner. Levels of endocannabinoid precursors were also generally down-regulated, indicating that KO affects levels of endocannabinoids in part by reducing the availability of their biosynthetic precursors. Usually smaller effects were found of KO on OEA and PEA levels. Conclusions: Our data suggest that KO may promote therapeutic benefit by reducing endocannabinoid precursor availability and hence endocannabinoid biosynthesis.
ABSTRACT: BACKGROUND: Omega-3 polyunsaturated fatty acids (omega-3-PUFA) are known to ameliorate several metabolic risk factors for cardiovascular disease, and an association between elevated peripheral levels of endogenous ligands of cannabinoid receptors (endocannabinoids) and the metabolic syndrome has been reported. We investigated the dose-dependent effects of dietary omega-3-PUFA supplementation, given as krill oil (KO), on metabolic parameters in high fat diet (HFD)-fed mice and, in parallel, on the levels, in inguinal and epididymal adipose tissue (AT), liver, gastrocnemius muscle, kidneys and heart, of: 1) the endocannabinoids, anandamide and 2-arachidonoylglycerol (2-AG), 2) two anandamide congeners which activate PPARalpha but not cannabinoid receptors, N-oleoylethanolamine (OEA) and N-palmitoylethanolamine (PEA), and 3) the direct biosynthetic precursors of these compounds. Methods: Lipids were identified and quantified using liquid chromatography coupled to atmospheric pressure chemical ionization single quadrupole mass spectrometry (LC-APCI- MS) or high resolution ion trap-time of flight mass spectrometry (LC-IT-ToF-MS).

Eight-week HFD increased endocannabinoid levels in all tissues except the liver and epididymal AT, and KO reduced anandamide and/or 2-AG levels in all tissues but not in the liver, usually in a dose-dependent manner. Levels of endocannabinoid precursors were also generally down-regulated, indicating that KO affects levels of endocannabinoids in part by reducing the availability of their biosynthetic precursors. Usually smaller effects were found of KO on OEA and PEA levels. Conclusions: Our data suggest that KO may promote therapeutic benefit by reducing endocannabinoid precursor availability and hence endocannabinoid biosynthesis.
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