Axnner, E., T. Gustavsson, et al. (2008). "Estradiol measurement after GnRH-stimulation as a method to diagnose the presence of ovaries in the female domestic cat." Theriogenology 70(2): 186-191.
Spaying is routinely recommended for population control in cats, as well as for specific health benefits and to avoid unwanted behaviors. It can be difficult to determine if a queen with an unknown history and no estrous activity is spayed or intact. In addition, one of the most common feline reproductive problems encountered by practicing veterinarians is ovarian remnant syndrome. A test that differentiates intact from spayed queens would be valuable. A commercially available luteinizing hormone assay (Witness LH, Synbiotics) has been marketed for this purpose, but is not available world-wide. GnRH induces release of follicle-stimulating hormone (FSH) and LH from the pituitary gland. FSH stimulates estradiol production by granulosa cells in ovarian follicles. The aim of this study was to evaluate if it is possible to differentiate between spayed and sexually inactive intact queens by measurement of plasma estradiol before and/or after stimulation with a GnRH-analogue, buserelin (Receptal, Intervet). Two groups of female cats (11 spayed, 11 intact) were treated with buserelin after baseline measurement of plasma estradiol and progesterone. A second blood sample was collected two hours later. Median estradiol increased after stimulation with buserelin in intact but not in spayed females. There was no overlap between the two groups of cats. Measurement of plasma estradiol concentration 2 hours after stimulation with a GnRH-analogue seems to be a reliable method to diagnose the presence of ovarian tissue in the female cat.
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